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human hcc cell lines hepg2  (ATCC)


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    ATCC human hcc cell lines hepg2
    Human Hcc Cell Lines Hepg2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 29964 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hcc+cell+lines/Hep+G2/10__1158_slash_2767___9764__crc___26___0139-76-5-32
    Average 99 stars, based on 29964 article reviews
    human hcc cell lines hepg2 - by Bioz Stars, 2026-10
    99/100 stars

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    Expressing:

    Article Title: Targeting CXCR6 Disrupts β-Catenin Signaling and Enhances Sorafenib Response in Hepatocellular Carcinoma.
    Article Snippet: .. Human HCC cell lines (SK-Hep-1, Hep-3B, SNU-398, JHH-2, and JHH-5) were obtained from ATCC or JCBR Cell Bank based on reported CXCR6 or b-catenin expression. ..

    Cell Culture:

    Article Title: Enzyme-Targeted Antiproliferative Effects of Novel Indole-Acrylamide Xenobiotics Acting on Cyclooxygenase Pathways.
    Article Snippet: .. Cell Culture The anticancer effects of all final compounds 6a–6dwere tested in vitro against eleven cancer cell lines, including human HCC cell lines (Huh7:JCRB0403, Hep3B: ATCC‐HB‐ 8064, HepG2: ATCC‐HB‐8065), Mahlavu [30] and SNU475: ATCC‐CRL‐2236), colon cancer cell lines (HCT116: ATCC‐CCL‐247) and MCF‐7: ATCC HTB‐22), adenocarcinoma (HeLa: CCL2) andCaCo‐2: CRL‐2102), normal human embryo kidney (HEK 293T: CRL‐3519), and mouse melanoma (B16‐F1: CRL‐6323). ..

    Article Title: Guaianolide dimer KGA-1002 targets GRP94 and triggers unfolded protein response-associated degradation of SKP2/AKT axis as a novel antihepatoma agent.
    Article Snippet: .. Human HCC cell lines (SK-Hep-1, Huh7, HepG2, and Hep3B) and the HEK-293T cells line were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), the immortalized human liver cell line (THLE-2) was purchased from the American Type Culture Collection (ATCC) (Rockville, MD, USA), and cultured according to the instructions. ..

    Article Title: Comprehensive multi-omics analysis reveals RUNX1's prognostic value, immune associations, and MUC13-Mediated mechanistic role in hepatocellular carcinoma pathogenesis
    Article Snippet: .. Human HCC cell lines (LO2, H9c2, HepG2, MHCC97-H, THLE2, and Huh7) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in DMEM supplemented with 10 % FBS and 1 % penicillin–streptomycin at 37 °C with 5 % CO 2 . ..

    Article Title: Quercetin suppresses the progression of HBV-associated hepatocellular carcinoma by modulating the EGFR signaling pathway.
    Article Snippet: .. Human HCC cell lines (HepG2 and Huh-7), procured from the ATCC, were cultured in DMEM (Gibco) containing 10% FBS (Procell) and 1% penicillin/streptomycin (P/S; Gibco) at 37 °C with 5% CO2. .. The following chemicals were used in this study: quercetin (Herbest, HR20119B1), osimertinib (MCE, AZD-9291), and lenvatinib (Selleck, S116404).

    In Vitro:

    Article Title: Enzyme-Targeted Antiproliferative Effects of Novel Indole-Acrylamide Xenobiotics Acting on Cyclooxygenase Pathways.
    Article Snippet: .. Cell Culture The anticancer effects of all final compounds 6a–6dwere tested in vitro against eleven cancer cell lines, including human HCC cell lines (Huh7:JCRB0403, Hep3B: ATCC‐HB‐ 8064, HepG2: ATCC‐HB‐8065), Mahlavu [30] and SNU475: ATCC‐CRL‐2236), colon cancer cell lines (HCT116: ATCC‐CCL‐247) and MCF‐7: ATCC HTB‐22), adenocarcinoma (HeLa: CCL2) andCaCo‐2: CRL‐2102), normal human embryo kidney (HEK 293T: CRL‐3519), and mouse melanoma (B16‐F1: CRL‐6323). ..

    Article Title: Enzyme-Targeted Antiproliferative Effects of Novel Indole–Acrylamide Xenobiotics Acting on Cyclooxygenase Pathways
    Article Snippet: .. The anticancer effects of all final compounds 6a – 6d were tested in vitro against eleven cancer cell lines, including human HCC cell lines (Huh7:JCRB0403, Hep3B: ATCC-HB-8064, HepG2: ATCC-HB-8065), Mahlavu [ ] and SNU475: ATCC-CRL-2236), colon cancer cell lines (HCT116: ATCC-CCL-247) and MCF-7: ATCC HTB-22), adenocarcinoma (HeLa: CCL2) and CaCo-2: CRL-2102), normal human embryo kidney (HEK 293T: CRL-3519), and mouse melanoma (B16-F1: CRL-6323). ..

    Planar Chromatography:

    Article Title: HDAC7 aggravates malignant proliferation of hepatocellular carcinoma cells via the TRIM26/CBX4 axis.
    Article Snippet: alcohol intake, metabolic liver diseases (notably non-alcoholic fatty liver disease), and exposure to aflatoxins and aristolochic acid (Yang et al. 2019).. Pathologically, regardless of the underlying etiology, HCC typically progresses through liver fibrosis and cirrhosis before progressing to overt malignancy (Alawyia and Constantinou 2023).. Moreover, HCC exhibits high molecular and cellular heterogeneity, which contributes to the complexity of its diagnosis and treatment (Chan et al. 2024).



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    Identification and multi-level validation of TPI1 as a pivotal prognostic driver. (A) Lollipop chart showing the selection frequency of feature genes across 101 machine learning models, identifying TPI1 as a high-frequency core gene. (B) Univariate Cox regression analysis of candidate genes; TPI1 exhibited the most substantial Hazard Ratio (HR), characterizing it as a preeminent risk factor. (C) Expression profiling of TPI1 across malignant versus paracancerous tissues within the TCGA-LIHC discovery cohort (upper) and GSE14520 validation cohort (lower). (D) Kaplan-Meier overall survival curves comparing patients with high and low TPI1 expression in the TCGA-LIHC (top) and GSE14520 (bottom) cohorts. (E) Relative mRNA expression levels of TPI1 in the immortalized human hepatocyte line (MIHA) and <t>HCC</t> cell <t>lines</t> <t>(Huh7,</t> SMMC-7721) determined by RT-qPCR. (F) Representative Western blot images (left) and densitometric quantification (right) of TPI1 protein levels in MIHA, Huh7, and SMMC-7721 cells. GAPDH served as the internal loading control. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001.
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    Identification and multi-level validation of TPI1 as a pivotal prognostic driver. (A) Lollipop chart showing the selection frequency of feature genes across 101 machine learning models, identifying TPI1 as a high-frequency core gene. (B) Univariate Cox regression analysis of candidate genes; TPI1 exhibited the most substantial Hazard Ratio (HR), characterizing it as a preeminent risk factor. (C) Expression profiling of TPI1 across malignant versus paracancerous tissues within the TCGA-LIHC discovery cohort (upper) and GSE14520 validation cohort (lower). (D) Kaplan-Meier overall survival curves comparing patients with high and low TPI1 expression in the TCGA-LIHC (top) and GSE14520 (bottom) cohorts. (E) Relative mRNA expression levels of TPI1 in the immortalized human hepatocyte line (MIHA) and <t>HCC</t> cell <t>lines</t> <t>(Huh7,</t> SMMC-7721) determined by RT-qPCR. (F) Representative Western blot images (left) and densitometric quantification (right) of TPI1 protein levels in MIHA, Huh7, and SMMC-7721 cells. GAPDH served as the internal loading control. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001.
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    Identification and multi-level validation of TPI1 as a pivotal prognostic driver. (A) Lollipop chart showing the selection frequency of feature genes across 101 machine learning models, identifying TPI1 as a high-frequency core gene. (B) Univariate Cox regression analysis of candidate genes; TPI1 exhibited the most substantial Hazard Ratio (HR), characterizing it as a preeminent risk factor. (C) Expression profiling of TPI1 across malignant versus paracancerous tissues within the TCGA-LIHC discovery cohort (upper) and GSE14520 validation cohort (lower). (D) Kaplan-Meier overall survival curves comparing patients with high and low TPI1 expression in the TCGA-LIHC (top) and GSE14520 (bottom) cohorts. (E) Relative mRNA expression levels of TPI1 in the immortalized human hepatocyte line (MIHA) and <t>HCC</t> cell <t>lines</t> <t>(Huh7,</t> SMMC-7721) determined by RT-qPCR. (F) Representative Western blot images (left) and densitometric quantification (right) of TPI1 protein levels in MIHA, Huh7, and SMMC-7721 cells. GAPDH served as the internal loading control. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001.
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    SLC41A3 promotes HCC progression. (A) Western blot analysis confirming the knockdown efficiency of SLC41A3 in <t>Hep3B</t> and HuH7 cells transfected with shRNA targeting SLC41A3. (B) CCK-8 assay evaluating cell proliferation at specified time points after SLC41A3 silencing in HCC cells. (C) Colony formation ability of Hep3B and HuH7 cells was inhibited after SLC41A3 knockdown. (D) Transwell migration and invasion assays indicated impaired migration and invasion capabilities after SLC41A3 downregulation in both cell lines. (E) Wound healing assay showed reduced cell motility after SLC41A3 knockdown. Scale bar, 100 μm. Data are presented as the mean ± SD of three independent experiments. *** P < 0.001.
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    hepg2 human hcc cell line  (Procell Inc)
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    SLC41A3 promotes HCC progression. (A) Western blot analysis confirming the knockdown efficiency of SLC41A3 in <t>Hep3B</t> and HuH7 cells transfected with shRNA targeting SLC41A3. (B) CCK-8 assay evaluating cell proliferation at specified time points after SLC41A3 silencing in HCC cells. (C) Colony formation ability of Hep3B and HuH7 cells was inhibited after SLC41A3 knockdown. (D) Transwell migration and invasion assays indicated impaired migration and invasion capabilities after SLC41A3 downregulation in both cell lines. (E) Wound healing assay showed reduced cell motility after SLC41A3 knockdown. Scale bar, 100 μm. Data are presented as the mean ± SD of three independent experiments. *** P < 0.001.
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    Average 86 stars, based on 1 article reviews
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    Image Search Results


    Identification and multi-level validation of TPI1 as a pivotal prognostic driver. (A) Lollipop chart showing the selection frequency of feature genes across 101 machine learning models, identifying TPI1 as a high-frequency core gene. (B) Univariate Cox regression analysis of candidate genes; TPI1 exhibited the most substantial Hazard Ratio (HR), characterizing it as a preeminent risk factor. (C) Expression profiling of TPI1 across malignant versus paracancerous tissues within the TCGA-LIHC discovery cohort (upper) and GSE14520 validation cohort (lower). (D) Kaplan-Meier overall survival curves comparing patients with high and low TPI1 expression in the TCGA-LIHC (top) and GSE14520 (bottom) cohorts. (E) Relative mRNA expression levels of TPI1 in the immortalized human hepatocyte line (MIHA) and HCC cell lines (Huh7, SMMC-7721) determined by RT-qPCR. (F) Representative Western blot images (left) and densitometric quantification (right) of TPI1 protein levels in MIHA, Huh7, and SMMC-7721 cells. GAPDH served as the internal loading control. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001.

    Journal: Translational Oncology

    Article Title: Integrating spatial and single-cell transcriptomics via machine learning to characterize efferocytosis in hepatocellular carcinoma prognosis and immunotherapy

    doi: 10.1016/j.tranon.2026.102801

    Figure Lengend Snippet: Identification and multi-level validation of TPI1 as a pivotal prognostic driver. (A) Lollipop chart showing the selection frequency of feature genes across 101 machine learning models, identifying TPI1 as a high-frequency core gene. (B) Univariate Cox regression analysis of candidate genes; TPI1 exhibited the most substantial Hazard Ratio (HR), characterizing it as a preeminent risk factor. (C) Expression profiling of TPI1 across malignant versus paracancerous tissues within the TCGA-LIHC discovery cohort (upper) and GSE14520 validation cohort (lower). (D) Kaplan-Meier overall survival curves comparing patients with high and low TPI1 expression in the TCGA-LIHC (top) and GSE14520 (bottom) cohorts. (E) Relative mRNA expression levels of TPI1 in the immortalized human hepatocyte line (MIHA) and HCC cell lines (Huh7, SMMC-7721) determined by RT-qPCR. (F) Representative Western blot images (left) and densitometric quantification (right) of TPI1 protein levels in MIHA, Huh7, and SMMC-7721 cells. GAPDH served as the internal loading control. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001.

    Article Snippet: The human HCC cell lines (Huh7 and SMMC-7721) and the immortalized human hepatocyte line MIHA were procured from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

    Techniques: Biomarker Discovery, Selection, Expressing, Quantitative RT-PCR, Western Blot, Control

    SLC41A3 promotes HCC progression. (A) Western blot analysis confirming the knockdown efficiency of SLC41A3 in Hep3B and HuH7 cells transfected with shRNA targeting SLC41A3. (B) CCK-8 assay evaluating cell proliferation at specified time points after SLC41A3 silencing in HCC cells. (C) Colony formation ability of Hep3B and HuH7 cells was inhibited after SLC41A3 knockdown. (D) Transwell migration and invasion assays indicated impaired migration and invasion capabilities after SLC41A3 downregulation in both cell lines. (E) Wound healing assay showed reduced cell motility after SLC41A3 knockdown. Scale bar, 100 μm. Data are presented as the mean ± SD of three independent experiments. *** P < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Comprehensive characterization of SLC41A3 identifies it as an immune-related prognostic biomarker and therapeutic target in hepatocellular carcinoma

    doi: 10.3389/fimmu.2026.1861310

    Figure Lengend Snippet: SLC41A3 promotes HCC progression. (A) Western blot analysis confirming the knockdown efficiency of SLC41A3 in Hep3B and HuH7 cells transfected with shRNA targeting SLC41A3. (B) CCK-8 assay evaluating cell proliferation at specified time points after SLC41A3 silencing in HCC cells. (C) Colony formation ability of Hep3B and HuH7 cells was inhibited after SLC41A3 knockdown. (D) Transwell migration and invasion assays indicated impaired migration and invasion capabilities after SLC41A3 downregulation in both cell lines. (E) Wound healing assay showed reduced cell motility after SLC41A3 knockdown. Scale bar, 100 μm. Data are presented as the mean ± SD of three independent experiments. *** P < 0.001.

    Article Snippet: The human HCC cell lines Hep3B and Huh7 were purchased from Procell Biotechnology.

    Techniques: Western Blot, Knockdown, Transfection, shRNA, CCK-8 Assay, Migration, Wound Healing Assay